|
|
Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
28/12/2011 |
Data da última atualização: |
28/12/2011 |
Autoria: |
SILVA, A. C. da; HIGUCHI, P.; BERG, E. van den; NUNES, M. H.; SANTOS, M. de C. N. |
Título: |
Variação espaço-temporal da dinâmica da comunidade arbórea em fragmentos de floresta aluvial em Minas Gerais. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Cerne, Lavras, v. 17, n. 4, p. 465-471, out./dez. 2011. |
Idioma: |
Português |
Palavras-Chave: |
Dinâmica florestal; Fragmento florestal. |
Thesagro: |
Mata Ciliar. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00604naa a2200193 a 4500 001 1911111 005 2011-12-28 008 2011 bl uuuu u00u1 u #d 100 1 $aSILVA, A. C. da 245 $aVariação espaço-temporal da dinâmica da comunidade arbórea em fragmentos de floresta aluvial em Minas Gerais. 260 $c2011 650 $aMata Ciliar 653 $aDinâmica florestal 653 $aFragmento florestal 700 1 $aHIGUCHI, P. 700 1 $aBERG, E. van den 700 1 $aNUNES, M. H. 700 1 $aSANTOS, M. de C. N. 773 $tCerne, Lavras$gv. 17, n. 4, p. 465-471, out./dez. 2011.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Florestas (CNPF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
Data corrente: |
16/11/2022 |
Data da última atualização: |
16/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
NICIURA, S. C. M.; OKINO, C. H.; NUCCI, A. DA S.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CINTIA HIROMI OKINO, CPPSE; ALESSANDRA DA SILVA NUCCI, Centro Universitário Central Paulista; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Polymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Parasitology Research, v. 121, p. 3581-3588, 2022. |
DOI: |
https://doi.org/10.1007/s00436-022-07682-6 |
Idioma: |
Inglês |
Conteúdo: |
Chemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus. MenosChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PC... Mostrar Tudo |
Palavras-Chave: |
Amino acetonitrile derivatives; Anthelmintic resistance; PCR. |
Thesagro: |
Haemonchus Contortus. |
Thesaurus NAL: |
Genotyping. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02402naa a2200265 a 4500 001 2148276 005 2022-11-16 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s00436-022-07682-6$2DOI 100 1 $aNICIURA, S. C. M. 245 $aPolymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus.$h[electronic resource] 260 $c2022 520 $aChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus. 650 $aGenotyping 650 $aHaemonchus Contortus 653 $aAmino acetonitrile derivatives 653 $aAnthelmintic resistance 653 $aPCR 700 1 $aOKINO, C. H. 700 1 $aNUCCI, A. DA S. 700 1 $aMALAGO JUNIOR, W. 700 1 $aBENAVIDES, M. V. 700 1 $aESTEVES, S. N. 700 1 $aCHAGAS, A. C. de S. 773 $tParasitology Research$gv. 121, p. 3581-3588, 2022.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|